Sentence examples for conventional cloning methods from inspiring English sources

All plasmids were constructed and propagated using conventional cloning methods [62].

Plasmids were constructed by conventional cloning methods.

Conservative site-specific recombinases offer an efficient alternative to conventional cloning methods.

To eliminate the problems of conventional cloning, methods avoiding the use of Type II restriction enzymes have been developed.

Following conventional cloning methods, unique restriction sites need to be added to all fragments (see Fig. 2 for example enzymes).

The CAGGS Fc expression vector was assembled by conventional cloning methods and is flanked by two attB sites (ɸC31 integrase recognition sites).

Two independent measurements (conventional cloning method and 454 sequencing) were performed, generating identical values.

Production at 30°C from the two best clones were then compared in a final spot blot, with the starting pool as well as with the best cell population (4-B4) obtained from the conventional cloning method described above (Fig. 3B).

The key advantage of our approach is the independence of the insert DNA sequence, which can - in contrast to the conventional cloning method - internally carry the recognition sequences of the restriction enzymes used for the digestion of the target plasmid vector.

Although intein methods can greatly simplify protein purification, commercially available expression vectors still rely on conventional restriction/ligation cloning methods for target gene insertion.

However, the susceptibility gene has not yet been identified because the chromosomal region has an extremely complex genomic structure with multiple gene duplications and inversions that have hampered conventional gene cloning methods [ 7].