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PCR primers (available upon request) were designed for the specific amplification of region B insertion junctions and conventional chromosomes, similar to those of S288C.
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In conventional chromosome preparation protocols, a spindle poison (e.g., colchicine) is employed to arrest the cells at their metaphase stage (Kligerman and Bloom 1977).
Meiotic chromosomes were prepared from the testes of adult male mice by conventional chromosome preparation [20].
Therefore, male meiosis was investigated by the analyses of testes sections and conventional chromosome preparations.
Previous study showed that gaps mainly existed in the heterochromatic regions and it was time-consuming to close the gaps by the conventional chromosome walking approach because of repetitive sequences [31].
EHCO carried out the conventional chromosome analysis of C. c. rubicundus.
Conventional chromosome analysis is laborious, however, and requires highly trained personnel.
Cytogenetic data based on conventional chromosome analysis and FISH were available for 108 (74.0%) patients.
PJSA carried out the conventional chromosome analysis of P. irrorata and performed the cladistic analysis.
Patients with hypodiploidy or −13 by conventional chromosome analysis were regarded as high risk.
Karyotyping by a conventional chromosome aberration analysis using Quinaqcrine-banding was performed after, typically, a few-week-long primary culture.
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