Sentence examples for convenient restriction from inspiring English sources

Convenient restriction sites allow for the facile introduction of additional amino-terminal domains.

To provide convenient restriction enzyme sites for recombinant plasmid construct, NdeI/XhoI sites were inserted into the 5′- and 3′-ends of the mu-IFN-CSP gene.

Since recombineering is directed by sequence homology, not convenient restriction sites, it can be used to create genetic alterations on large DNA molecules such as bacterial artificial chromosomes or bacterial genomes in a way not possible with classical in vitro genetic engineering techniques.

Since recombineering is directed by sequence homology, not convenient restriction sites, it can be used to create genetic alterations on large DNA molecules such as bacterial artificial chromosomes or chromosomes in a way not possible with classical in vitro genetic engineering techniques.

By substituting Y with G, it was possible to introduce convenient restriction enzyme site (SmaI), thus allowing screening of mutants.

For this complex gene library, no convenient restriction sites could be found for cloning into the modified pUC19 expression vector without cutting a fraction of the insert sequences.

DNA fragments were cloned into expression plasmids using convenient restrictions sites and all clones used in this study were sequenced to ensure the absence of secondary undesired mutations.

Briefly, NarI and EcoRI digestion (convenient and unique restriction enzyme cloning sites in both pCR-XL-TOPO and pBR-SK9) was performed to allow subcloning of near full-length proviral DNA into the background of p93JP-NH1.

The normal E1A regulatory elements have been replaced by a convenient set of unique restriction enzyme sites, allowing for introduction of gene regulatory cassettes with relative ease.

We initially employed the pBS/U6 vector (now sold as pSilencer 1.0-U6, available from Ambion, Austin, TX) which contains the RNA PolIII-specific U6 gene promoter along with restriction sites convenient for oligonucleotide insertion.

These sequences were then assembled into the synthetic operon in silico, with the concomitant introduction of ribosome binding sites and restriction sites for convenient downstream manipulation.