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On one end, the linker has an NHS group that can be coupled with any amine, such as peptides, proteins or amine-linkers, and on the other end an alkyne for convenient attachment to materials with an azide functional group.
As shown in Fig. 1a, the linker 1 can be photocleaved with UV light (365 nm), possesses an NHS-ester (N-Hydroxysuccinimide ester) functional group to react with an amine of choice, and possesses an alkyne group for convenient attachment to any material with an azide functional group by using CuAAC (copper catalysed azide alkyne cycloaddition, also known as the "click reaction").
Although no stability data were reported for this method, its potential application in the convenient attachment of rHRP onto gold electrodes for biosensing is obvious.
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Although convenient, covalent attachment and co-polymerisation require substantial amounts of protein to obtain a good response [ 11].
Linking carboxylate, isothiocyanate or maleimide groups to the terminal amine of H3DFO (e.g. Chart 1) provides a convenient, stable attachment point for conjugation to amino acids of antibody side chains.
She wants "to make a sensor that is as accurate as what's on the market now, but much more convenient, perhaps an attachment to an iPhone or tablet," she says.
The eight-carbon side chain of this residue mimicked the N-terminal fatty acyl chain of polymyxin B. To avoid the need for additional orthogonal protection during the synthesis, the N-α-amino group was employed as a convenient point of attachment for the dansyl group.
The snap button releases for attachments hold everything securely in place, too, and you get the convenient wall mount and attachment holder in the box, too.
The intact Au surface provides convenient anchorage sites for attachment of targeting molecules, and the particles can be activated by both near infrared lights and magnetic fields.
Attach lower attachment assembly.
They also plan to integrate the new media search with their iGoogle widget, which should make finding photos and attachments even more convenient.
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