All patients were managed with standard diet conservative treatment (LPD) according to (Italian Society of Nephrology) SIN.42 Controls were carried out at baseline (T0), after 3 weeks (T1) and after 6 weeks (T2).
As previously described (Battifora, 1991; Decaestecker et al, 2009), additional technical and fixative controls were carried out by staining the TMA slides with haematoxylin eosin and anti-vimentin (V-9, dilution 1 100; BioGenex), respectively.
Both controls were carried out in triplicate.
Controls were carried out under identical conditions without copper sulphate.
Negative controls were carried out without lipase or fatty acid.
All experiments (treatments and controls) were carried out triplicate and all data were expressed as the mean ± SD.
All experiments (treatments and controls) were carried out for three times and the obtained data were reported as the mean ± SD.
All assays were performed in triplicates, and one no-template control and two interplate controls were carried along in each experiment.
Quality controls were carried out daily.
Negative extraction controls were carried through subsequent PCR and tRFLP analyses.
Several controls were carried out to assess antibody specificity and nonspecific immunoreactivity.
