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Hexon-modified vectors, as well as controls vectors, were subjected to heating at 45°C for various time points (0,5,10, 20 and 45 minutes).
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We can briefly separate vector control into two prevailing forces: targeted vector control versus widespread vector control.
Control vectors were ECFP, EGFP, EYFP, and DsRed-monomer.
Details of our shRNA and control vectors, as well as our infusion procedures, are provided in Cozzoli et al (2012).
(a) qPCR analysis showing NAP1L3 mRNA levels (normalised to UBC) in sorted (Lin−CD34+CD38−) UCB HSCs transduced with shRNA vectors targeting NAP1L3 (NAP1L3 shRNA) or control vectors (SC shRNA).
Negative control vectors targeting GFP or containing a non-targeting sequence are also available.
Control vectors had no insert.
Control vectors harboring GFP cDNA (Ad-GFP) were produced using the Adeno-X system.
All cis2 promoter-less control vectors showed similar background activity (data not shown).
Transfection efficiencies were estimated at approximately 60% using GFP-containing control vectors.
The cells transfected with control vectors showed no aggregates and apoptosis.
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