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Our experiments were designed to identify suitable housekeeping genes (HKGs) in disk abalone as internal controls to quantify biomarker expression following endocrine disrupting chemicals (EDCs).
The selected reference genes were used as internal controls to quantify CBSV in various symptomatic and asymptomatic plant organs to establish a correlation between virus load and symptom severity.
We used these differences between both sets of healthy samples (British 1958 Birth Cohort cell lines and Turkish controls) to quantify the reduction in diversity in the transformed cell lines.
Reference controls to quantify the relative contents of Pgp and Mrp1 were obtained by serial dilutions of pooled microvessels and 4th ventricle choroid plexuses obtained from P60 Jj Gunn rats and run for each Western blot procedure (Fig. 1).
The expression of the nuclear genes, used as internal controls to quantify mtDNA content in qPCR assays, was not significantly associated with PM10 exposure during the various time windows (p > 0.28).
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Conservative tracer studies of bromide were conducted as a control to quantify the deposition of colloids onto grass surfaces and the mass exchange of colloids between the overland flow and soil underneath under various experimental conditions.
The Tubulin transcript was used as an internal control to quantify the relative transcript levels.
Whereas the "housekeeping" RpL8 gene represents basically per-cell transcripts comparison, OR7 gene might represent a more suitable control to quantify olfactory-specific transcripts ratios considering the structure of Cx. quinquefasciatus antennae.
GAPDH transcripts were monitored as a control to quantify the transcripts of the genes in each sample.
S-2302 was used to quantify FXIIa, and S-2238 as a control to quantify thrombin since thrombin also can cleave S-2302.
Parallel transduction using a GFP-encoding virus served as a control to quantify infection efficiency (usually >90%) and treatment related cell death (usually ~ 20%).
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