Sentence examples for controls template from inspiring English sources
Negative controls (template consisting of ultrapure water) were run for each gene.
No-template PCR controls (consisting of master mix, primers, and water) and sham digest controls (template consisting of water subjected to DNA extraction) were run with each PCR assay to monitor for contamination.
Negative DNA controls (template DNA replaced with distilled water) and positive controls (Acanthamoeba lenticulata ATCC30841) and sample DNA were analyzed in triplicates during each PCR run.
Thus, a total of 37 reactions were studied per reference gene per tissue, including one negative control (template control without cDNA).
Positive control (template provided with kit) and negative control (heat inactivated samples) reactions were performed.
A negative control (template replaced by Nuclease-free water) was included in each PCR run.
Rigorous assay characterization also required a standard control template.
The suspension was centrifuged and processed as described for DNA extraction for positive control template DNA.
PolyA+ submitted to reverse transcription without the actual RT enzyme were used as negative control template.
Control template strands contained unmodified dT at position X.
The clone named MF12 was used as the positive control template for PCR reactions.
