Suggestions(1)
Exact(10)
As controls, reactions were performed in the absence of LRRK2 protein or ATP.
Total RNA extracted from human brain was used for positive controls; reactions lacking RT were also included.
As for light scatter assays, controls reactions contained an equivalent volume of ClpX storage buffer instead of the ClpX protein.
As controls, reactions containing substrate without infected BH seed were also subjected to cycling and samples containing substrate and infected BH were held at 37°C without cycling, but neither showed amplification.
Controls reactions in the absence of reverse transcriptase were used verify the absence of DNA contamination.
All of the appropriate blanks and controls reactions were performed as described in [ 25].
Similar(50)
There were no differences between CP participants' and controls' reaction times.
All control reactions are represented as empty marks.
"NC" indicates negative control (reactions with no PCR template).
Use of proteasome inhibitor (MG132) in control reactions ensured the specificity of the enzymatic reaction.
Cell control reactions lacked the silver salt and salt controls lacked the biomass.
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