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There were no differences between CP participants' and controls' reaction times.
For each gene, three repeated experiments, including internal controls and negative controls (reaction samples without cDNA templates), were conducted.
For qRT-PCR, three biological replicates were performed for each sample plus template-free samples and other negative controls (reaction without reverse transcriptase).
For each selected gene, triplicate sets of PCR reaction samples including the 18S controls, and duplicate negative controls (reaction samples without cDNA templates), were prepared and run in a 96-well plate.
For the typically developing controls, reaction times were significantly longer for ED1 trials compared with ED3 trials (P < 0.02), but there was no significant difference between ID1 and ID3 trials (P = 0.54).
Similar(54)
As controls, reactions were performed in the absence of LRRK2 protein or ATP.
His out-of-control reaction can be explained, but not justified.
You really can't control reaction, you can't control sales.
A blank: the absorbance of the control reaction.
A control reaction was also processed with heat denatured enzyme.
Unfortunately, attempts were failed to improve the yield of 11 by controlling reaction time and temperature.
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