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Whereas NGF, BDNF, TrkB, TrkC, p75NTR and sortilin were detected in TASMCs from patients and controls, proteins for TrkA receptor were not detected in cells from either of these groups.
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To further investigate the effect of rIDE on VZV infectivity, we incubated cell-free VZV ROka-lacZ with rIDE or control proteins for 15 min at 37°C before infecting human melanoma cells with the virus.
Cell-free zoster vaccine virus, which contains various stabilizers, was reconstituted according to the manufacturer's instruction, and incubated with rIDE or control proteins for 30 min at 37°C.
Three non-transmembrane proteins were also studied as another set of control proteins for this procedure.
We assayed several control proteins for their effect on Lipid II topology.
We also selected several control proteins for the validation of the quantification method (ACAN, COL2A1, TG2, CTSB, CTNNB1).
Of proteins identified by proteomic analysis, we selected 39 proteins (30 candidate GRPs and 9 control proteins) for further analysis using proteomic and bioinformatic approaches (see Results section).
Three transmembrane proteins; transferrin receptor, aquaporin 1 and synaptotagmin 1, were studied as a set of control proteins for the classical procedure of alkaline sodium carbonate extraction.
To the best of our knowledge, this is the first study to identify these loading control proteins for thyroid cancer specimens.
As there are no reliable internal control proteins for Western blot analysis of the serum samples, a loading control sample was generated by pooling all of the samples from all groups in equal volumes (20 μL) for each gel.
The pseudo wild-type template (C290A, C393A, 11) was then further mutated to produce singly phosphorylated threonine (12) and serine (13) variants at position 288 as control proteins for in vitro biochemical analyses.
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