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The GAPDH RT PCR products were used as controls of sample loading for normalisation between samples.
LOXL2 was significantly elevated in PDAC versus healthy controls of Sample Set A (p = 0.019, Table 2); however this marker showed no significant difference in the comparisons between the other groups.
PDAC versus non-cancer samples from Sample Set B was used as a training set from which models were derived and then validated in the PDAC versus healthy controls of Sample Set A. Models for early-stage PDAC compared to healthy controls were also assessed.
aBiomarker models for two and three marker combinations generated in PDAC versus disease-free controls of Sample Set B and presented in Table 3 were validated in PDAC (n = 100) versus healthy controls (n = 92) of Sample Set A and ordered from greatest to lowest AUC.
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Microcapillary injector geometries are being designed to allow increased control of sample plug volumes.
The control of sample positioning mechanism, stimulation system, precise timing and photon counting are accomplished using entirely this board.
Precise control of sample volume is one of the most important functions in lab-on-a-chip (LOC) systems, especially for chemical and biological reactions.
GAPDH was used as the loading control of sample by reprobing with an anti-GAPDH antibody (1 4000, Santa Cruz Biotechnology).
GAPDH was used as the loading control of sample by reprobing with an anti-GAPDH antibody at a 1 12000 dilution.
The imposed diet and health education were used as control measure, for avoiding contamination of the sample, and for producing better control of sample studied.
Due to the limited number of samples i.e. 3 animals per time point and tissue, the control of sample variability is crucial.
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