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Primers for human β-globin were used as positive controls of human DNA amplification (3 ).
Negative controls of human hepatocellular cancer remained negative for every tissue sample (N=39, not shown).
Molecular mass standard (ColorBurst, Sigma-Aldrich) containing the precisely sized recombinant proteins of 210, 90, 65, 40, 30, 20, 13, 8 kda, positive controls of human recombinant TNF- α (80 ng) or human recombinant IFN- γ (80 ng) (R&D systems), and the samples of the SNpc were run in the same gel and transferred onto a nitrocellulose membrane.
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