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By using these potential alpha-synuclein up-regulators, we have identified an SNCA CpG island that controls in vitro alpha-synuclein expression, and then analyzed postmortem brain tissues to find PD-specific SNCA promoter demethylation in the substantia nigra.
For positive and negative controls, in vitro methylated DNA (IVD) and H2O were treated, respectively.
This provides an explanation for the higher proliferation rates of DM fetal myoblasts than controls in vitro [ 8, 14].
As controls, in vitro phosphorylation experiments of CagA with various cell lysates indicated that the kinases are active and strongly phosphorylated CagA [ 74].
Compared to controls, in vitro enzymatic activity assays of affected patients' erythrocytes and cultured fibroblasts have a 44%-45 44%-45ase in PRS-I activity [ 5].
Compared to controls, in vitro enzymatic activity assays in affected patients' cultured fibroblasts have a 62% decrease in PRPS1 activity [ 5].
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In comparison, the control in-vitro-cultured NRP-1 knockdown MSCs produced highly disorganized network assemblies (results not shown), as previously demonstrated (see Figures 7B and 7D).
Design: Prospective, controlled, in vitro study.
A promising VLP production route is the controlled in vitro assembly of virus proteins into capsids.
By introducing and disrupting synchrony, we could activate or deactivate the limiting length control and speed control in vitro in the solution and we provide live imaging how each molecule comes nearby and assemble.
Additionally, controlling the cell-dose within the biomimetic capsules enables a controlled in vitro and in vivo drug delivery.
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