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Two identical sub-arrays were printed per slide with each sub-array comprising 352 spots (80 glycans and 8 controls in replicates of 4).
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Each concentration was tested in triplicate and controls were performed in replicates of eight.
To control for variability, each ECMP combination was printed in replicates of five spots.
Nominal concentrations of 6, 20, 60, 200 and 600 mg PSOA/L were prepared in replicates of five, in addition to a control.
Early in tumorigenesis (at 2 months of age), 46 probes representing 40 unique genes were significantly up-regulated and 86 probes or 73 unique genes were significantly down-regulated in the HMGA1 transgenics compared to controls in replicate samples by the nonparametric correlative analysis of microarrays (CAM) approach [ 41].
In a field in which designing controls and performing replicates of experiments is crucial for building scientific understanding, one might expect that the assessment tools developed by biology educators would also contain reliability measures.
Only those genes whose expressions were significantly modulated (i.e., 1.5-fold and p<0.05, when compared to those of mock-infected controls) in all of the replicates (N = 3) analyzed at each time point were selected for further investigation.
Standard sample and negative control were run in three replicates of a 96 well-plate.
A transcript downstream of AT1G02360 shows flg22-dependent downregulation (see Additional file 1, Figure S4A), while a chloroplast-derived transcript upstream of ATCG00270 (see Additional file 1, Figure S4B) shows similar and high expression in all replicates of control and treatment samples.
Negative controls (in the absence of peptide) were prepared in eight replicate samples.
Control mortality in the three replicates of A. ferus eggs ranged from 47.5 to 52.5%.
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