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Cyclophilin or HPRT mRNA were assayed as controls in real-time PCR assays.
Normal bone marrow CD34+ CD19+ progenitors from normal bone marrow donors were used as controls in real-time PCR experiments.
Samples without reverse transcriptase served as negative controls in real-time PCR to exclude genomic DNA contamination.
These two genes are widely used as internal controls in real-time PCR [ 28]; yet both may be under transcriptional regulation in time and/or space in an actively growing organ.
We chose two M. brunnea NLP homologous genes (MbNPP1 and MbNPP2), which are homologous to BcNep1 and BcNep2 of Botrytis cinerea [ 36], as controls in real-time RT-PCR experiments.
The high regulation of Actin11 and EF1β expression and their patterning along root growth zone pointed out that these genes did not display the expression stability required for their use as endogenous controls in real-time PCR.
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For these reasons an in-house pneumatically actuated MR-compatible robot was developed where needle-guide direction can be controlled in real-time inside the controller room [ 4].
A robot is controlled in real-time based on predicted pedestrian trajectories using the proposed AR-GP motion controller.
For measure and control in real-time there was used IO card from Advantech, embedded PC and operating system Linux.
In addition, the modeling and control process are very concise, and they can be easily applied in various power classes PEMFC temperature control in real-time.
The project summarized in this article aims at developing techniques to support access control in Real-Time Distributed Collaborative Editors (RCE).
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