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A dengue virus targeting IgG1-12A1 and a hexa-histidine-tagged anti-RANK receptor binding scFv were used as isotype controls for the experiments.
Controls for the experiments consisted of the omission of primary antibodies.
No RT control (without the reverse transcriptase) and a no template control (without the RNA template) were taken as the negative controls for the experiments.
As controls for the experiments, similar single-cysteine substituted mutants at amino acid positions 107 135 were generated on the background of wild type (WT) rhodopsin, containing the six natural cystein residues, which are labeled as P107C R135C/WT.
As controls for the experiments described above, no immunostaining was observed in DRG neurons or spinal cord when using preimmune serum or when the chicken anti-PAP antibody was omitted (data not shown).
Controls for the experiments consisted of the omission of primary antibodies or agglutinin from the procedure.
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Cells treated with low-dose ionizing radiation (IR) were used as positive controls for the experiment.
Healthy animals (HEALTHY) underwent no anesthesia and no surgery and were considered as controls for the experiment.
Our cell lines 'healed' scrapes steadily over the course of 24 27 hours (micrographs of the process are shown in the controls for the experiment in Fig. 4).
Cells were transfected as before and treatments with H2O2 or the mitochondrial complex I inhibitor 1-methyl-4-phenylpyridinium (MPP+) iodide served as positive controls for the experiment.
While we expected to obtain sequence changes primarily at position 748, randomization of residues 746 and 747 served as, respectively, positive and negative controls for the experiment.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com