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Controls, for each time point, were also set up.
Humans set the parameters and establish the controls for each virtual environment.
The specificity of the GeXP assay was examined using positive controls for each virus.
Controls for each enzyme were run that received 50%% methanol in lieu of any cyanobacterial extract.
Furthermore, "no reverse transcription" and "no template" samples were included as negative controls for each reaction.
The latter two cassettes were used as normalization controls for each experiments.
U2OS cells containing the empty cloning vector U2OS-pSG2133) were used as controls for each condition.
All reactions including no-template and RT minus controls for each miRNA were run in triplicate.
Single-stained controls for each fluorochrome were used for compensation settings.
Probe intensities were background corrected by subtracting the mean of the negative controls for each array.
Other cells in the sections served as internal controls for each antibody.
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