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Pseudomyxoma peritonei cell cultures were established from intra-abdominal diseased tissue, paired with controls cultured from skin obtained from tissue excised around the laparotomy scar.
We also used four controls (cultured fibroblasts from healthy skin samples).
RNA from fibroblasts from three subjects with HGPS and three controls, cultured with or without FTI, was used.
Aggregates cultured in FGFs 1, 4 or 8, however, showed differentiation outcomes indistinguishable from controls cultured in serum-free differentiation medium (Figure 4A C).
EPL cells maintained in serum-free differentiation medium formed neurons efficiently (65% to 85%) when compared to controls cultured in serum-containing medium (<8%).
Table S1 lists the primer sequences of 56 selected genes that were analyzed by qPCR on the 21 cell lines from patients and controls, cultured without stimulus or exposed to Th1 or Th2 cytokines.
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Compared to the control cultured lenses, expression of GRP78/Bip in xylose cultured lenses was significantly induced (Figure 12).
RNA was collected from CTGF and control cultured ovaries as described in the Methods from three replicate experiments.
In control cultured cells, UPS-positive PaCSs were observed only occasionally and in the absence of any H. pylori product-related reactivity.
As a control, cultured bone marrow-derived macrophages were used.
Control cultured embryos were treated with the same amount of DMSO.
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