Sentence examples for controls as previously from inspiring English sources

The distribution of CMS-ICC was evaluated via a fluorescence microscope on formalin-fixed, paraffin-embedded, 4′,6-diamidino-2-phenylindole (DAPI) counterstained sections from all tissues in groups 1, 3, and 5 (Table 1) while the respective vehicle treated groups 2, 4, and 6 served as negative controls as previously described [22].

Parasites starved with 3 mM glucose were used as controls, as previously described.

BMDMs were generated from 6- to 8-week-old IL-16−/− C57BL/6 and littermate controls, as previously described [43].

Hydroxytamoxifen-dosed mice with floxed Cdk5 alleles mice served as WT littermate controls, as previously reported [7].

Micro-CT was performed in total body and femur of Lmna−/− mice and WT controls as previously described [10].

Fasting expression of the PI3K p85α and p110β subunits, PKCζ and GLUT4 was reduced in LBW subjects when compared to controls, as previously reported (8) (Figure 1).

ARP was used as a housekeeping expression control as previously described [31].

Neonatal mice were injected with vector or saline control as previously described [48], [54], [55].

Two µg of dominant active RhoA (RhoA DA) in pcDNA3.1 was transfected as a positive control, as previously described [41].

Prior to the analysis, we performed genotype calling and quality control as previously described in Cho et al. [39].

The relative quantity of target genes was calculated using the comparative threshold method (ΔΔCt) and using Ppib as endogenous control as previously described[55].