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If cases and controls are run in separate batches, genuine biological variation can be entirely confounded by batch effects.
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Negative controls were run in the absence of the anti-CD147 primary antibody.
Controls were run without peptides.
All treatments including controls were run in quadruplicates.
All samples, standards, and controls were run in triplicate.
Negative controls were run without primary antibody incubation.
Standard curves, melting curves and negative controls were run for each qPCR run.
All reactions, including the no-template controls, were run in triplicate.
All of the reactions, including the no-template controls, were run in triplicate.
Positive viral samples and negative controls were run individually for each assay.
Two controls were run along with the test tissue samples from transgenic plants.
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