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Collectively, the data demonstrate that PRV provides a powerful means of dissecting the synaptology of CNS circuitry when appropriate controls are incorporated into the experimental design.
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Additional positive and negative controls were incorporated in every antimicrobial activity assay plate, including each antimicrobial compound concentration in the absence of bacteria, bacteria in the absence of antimicrobials, and media in the absence of bacteria or antimicrobials.
Virus controls were incorporated after the longest exposure time (60 s).
Virus controls were incorporated after the longest exposure time.
Appropriate positive controls to help with orientation and vector controls were incorporated on the microrarray.
Positive (CVS-27 and EBLV-1) and negative (cell culture medium) controls were incorporated in each test.
Finally, 12 clinical studies containing 1,086 subjects (736 GC patients and 350 controls) were incorporated into the current meta-analysis.
In addition, 612 cases from a more recent meta-analysis and 1129 controls were incorporated as well [ 41].
To complete the 1536 spots available in the array (12 × 8 × 16), 100 positive and negative controls were incorporated.
Assay controls were incorporated onto the same plate, namely, no-template controls to test for the contamination of any assay reagents.
Controls were incorporated in triplicate by incubating fungal material without ant brains, ant brains without fungal material, and the medium without fungal material or ant brains.
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