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In general, the experiments need more controls and quantification to be interpretable.
Some of the experimental data (especially the imaging data) is lacking critical controls and quantification.
1) The in vitro experiments require additional controls and quantification: 1A) There is a huge difference in electrophoretic mobility between the endogenous oMAP4 and the recombinant protein used for reconstitution experiments.
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ChemStation Rev.A.10.02 (Agilent, UK) software was used to undertake instrument control and quantification.
After quality control and quantification, the library was pooled and enriched for 4813 genes associated to monogenetic disorders.
Glass argued that modern culture erodes students' ability to express vulnerability, encourages us to push aside "the other" in our pursuit of power, knowledge and status, and emphasizes control and quantification over trust and nuance.
This enables an efficient practical implementation of the FA protocol with control and quantification of the interference levels, while not adding too much of an additional burden to the network communication overhead.
Yet the control and quantification of transient gene expression in unperturbed dividing cells has received less attention.
GAPDH was used as an endogenous control and quantification was performed using the relative quantification method where the real-time PCR signal of the experimental RNA was measured in relation to the signal of the control.
Genomic DNA extracted from L. interrogans serovar Lai strain Lai was used as a positive control and quantification calibrator for the estimation of bacterial copy number in clinical samples.
Instrument control and quantification were performed using Analyst 1.4.2 and MultiQuant software (AB Sciex Inc., Thornhill, ON, Canada).
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