All positive samples and at least 10% of negative samples were sent to INEVH for quality control and confirmation.
Following detection of GFRa1, membranes were stripped and probed for β-actin, which served as a loading control and confirmation that the supernatant fraction was not contaminated with cells or cellular debris (lower panel).
As controls and for confirmation, nuclear extracts from parallel cultures were analyzed by immunoblotting (Figure 3B).
Blood samples were collected either in slaughter plants or by qualified veterinarians through their routine practice, in the framework of official programs aimed at the identification, health control and parentage confirmation of the breeds and populations included in our study.
With appropriate replication and controls, and following the confirmation of genetic or plastic change and its adaptive significance (as above), exceptionally strong evidence thus would be provided for causal effects.
Similar studies also include another earlier report about miR-21 expression in ESCC; Hiyoshi et al. [ 14] also used qRT-PCR technique to detect the status of miR-21 in fresh ESCC tissues and paired controls, and as further confirmation, ISH approach was performed on FFPE blocks; it could localize gene expression to specific cell types in specific regions.
Due to the above mentioned limitations and the lack of certain controls and of in planta confirmation, the reviewers agreed that the manuscript is more suitable for a specialized journal.
Several genes, other than PRNP, have recently been found to be associated with a risk of developing sporadic or variant Creutzfeldt-Jakob disease, but these novel data have been produced in a relatively small number of patients and controls and, therefore, need further confirmation.
For quality control purposes, C. difficile culture and confirmation by immunoassay will be undertaken in 1 in 5 C.
Mid-June's power grab, including dissolution of the democratically elected parliament, institution of martial law and confirmation of budgetary control, appear long forgotten.
