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Controlling the microenvironment of such organotypic cultures has impact in tissue engineering, cancer therapy and personalized medicine.
These results demonstrate the importance of finely controlling the microenvironment of transplanted cells in the treatment of severe muscle damage.
Especially the intraluminal cues controlling the microenvironment of the conduits are in an infantile stage but there is profound potential in the application of the scaffolds.
Controlling the microenvironment of injected myogenic cells using biological scaffolds enhance muscle regeneration (Borselli et al. 2011).
The difference in exposure time necessary to elicit peptide release between K+ and insulin demonstrates that by further controlling the microenvironment, we can gain a greater understanding of the conditions necessary for peptide release to occur.
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Gene delivery from tissue engineering scaffolds provides the opportunity to control the microenvironment by inducing expression of regenerative factors.
We summarize examples where these tools have helped to control the microenvironment of cells, and have been useful in solving problems in fundamental cell biology.
By bioprinting neuronal constructs, one can precisely control the microenvironment by specifically formulating the bioink for neural tissues, and by spatially patterning cell types and scaffold properties in three dimensions.
Thus, zeolites can be used to control the microenvironment of enzymes [13].
A central concern of tissue engineering is to understand how to control the microenvironment surrounding the cells to restore similar conditions for cells as in their innate environment.
Moreover, failure to control the microenvironment of stem cells can also have deleterious effects such as dedifferentiation and subsequent tumor growth.
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