We undertook several approaches to control for reference genome bias in copy number estimation.
We discuss additional approaches to control for reference genome bias in more detail in connection with the relevant results below.
In order to control for reference bias and overalignment, each subtomogram was assigned starting Euler angles based only on the position of the subtomogram relative to the directional tube axis.
If a proportion of the A. americanum ESTs that failed to match the I. scapularis datasets is discarded to control for reference quality, higher values for homology between A. americanum and I. scapularis are estimated: 34% matching between A. americanum ESTs and the I. scapularis predicted peptides, 35% matching with I. scapularis contigs, and 43% matching with I. scapularis singletons.
Regression analyses tested whether, after controlling for its reference standard questionnaires, the STarT Back Tool (independent variable) predicted treatment-related changes in global improvement, pain severity, disability, catastrophizing, fear, and depression (dependent variables).
We used an analysis of covariance (ANCOVA) to test for differences in Hsp70 Ct values among genotypes within time points, controlling for the reference gene by including Act5C Ct as a covariate for each sample in the analysis.
After controlling for age, reference year, income, alcohol consumption, serum lipids, and serum DDE, we observed statistically significant inverse associations for PCBs 170, 196, and 201 with endometriosis risk.
Posterior classification probabilities are used to explore areas at divergent risk from the reference while controlling for multiple testing.
Untransfected parent cells were unreactive with any of the antibodies (Table 3, Parent column); shown here is the murine IgG2a irrelevant primary antibody control; normal rabbit IgG, controlled for concentrations of reference antisera, was also negative.
45 male Zucker rats (Harlan, United Kingdom) were used, comprising 36 obese rats (HsdOla:ZUCKER- Lepr fa, homozygous fa/fa) as the experimental model for the MS study, and 9 lean rats (heterozygous Fa/fa) as the healthy controls for reference values.
