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Second, in the mRNA expression studies, all data were normalized to GAPDH for the purpose of controlling for cell numbers.
However, when controlling for cell proliferation, the difference in the redox ratio between MCF7 and BT474 cells increased in the current study (Fig. 5).
Cellular invasiveness was measured by a QCM invadopodia assay, which examined degradation of a fluorescent gelatin matrix while controlling for cell number and size.
Genomic DNA was isolated from purified Teff and, separately, Treg cells, from each subject's samples using a DNeasy Mini Kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions and controlling for cell number.
In stage 1, after controlling for cell type composition in peripheral blood, the interaction term 'OCP use × rs1269486' was found to be associated with differential methylation of cg17124583 (P value = 0.002; FDR P value = 0.04; Table 4), indicating that this SNP represents a conditional methQTL.
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Similar experiments were further performed to control for cell density.
We therefore controlled for cell density, by treating C2C12 cells in GM for 24 hours with -JQ1, trypsinizing, counting, and then re-seeding equal numbers of cells for each experimental group in DM to induce differentiation (Fig. 4g).
We counterstained f-actin with Alexa-fluor 680-labeled phalloidin to control for cell numbers.
To control for cell synchronization, cells for the + N and –N conditions were harvested at the same time of day.
Through the use of cytometer gating, we controlled for cell size and morphology (singlets vs budding cells).
One flask was then used to control for cell competence for transfection by using a plasmid encoding GFP (pcDNA3-GFP).
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