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This is the first report of separate genetic loci controlling cell wall phenotypes above and below ground.
Since none of the identified WalKR regulon genes appear to be essential, this suggested that the WalKR requirement for cell viability could be specifically linked to its role in controlling cell wall metabolism.
Cell wall-loosening proteins are believed to play key roles in controlling cell wall extension [ 56].
Recently, the existence of such transcriptional regulators controlling cell wall integrity and plant growth was demonstrated [ 12, 13].
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Thus, inclusion of LytR in the Clades C/D SigB regulons suggests that controlling cell wall-specific functions is important for supporting invasive disease.
The PKC pathway activated by Rho1 is involved in actin cytoskeleton organization and the transcription of cell wall biosynthesis genes [14] [17] and Rho1 controls cell wall synthesis directly by activating the (1,3 β-glucan synthase FKS1,3 β-glucan.
The protein, RodZ, is required for assembly of the actin cytoskeleton MreB that controls cell wall synthesis and cell shape.
These regulatory genes might control cell wall digestibility spatially and temporally among the two groups of lines in the FF population.
This multi-domain integral membrane protein forms a complex that controls cell wall synthesis in M. tuberculosis upon phosphorylation by the serine/threonine kinase PknB [ 42].
Valentini et al. showed that eIF5A is involved in the WSC/PKC1 signaling pathway that controls cell wall integrity or related processes, and plays a role in cell wall formation [ 18].
Our hypothesis is that EXO integrates cellular, metabolic, and/or environmental factors, and feeds this information into an unknown signalling pathway which controls cell wall properties and metabolic pathways.
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Justyna Jupowicz-Kozak
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