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After the inlet and outlets reservoirs were sealed with Epoxy, the microfluidic device was placed on a heating film at 63 °C for 45 min for LAMP reactions, followed by the termination of LAMP reactions at 80 °C for 2 min. The heating film was controlled by an inexpensive proportional integral derivative (PID -based temPID -basedontemperatureised by our laboratory.
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