In addition, an easily controlled template-assisted growth method has also drawn significant interest because the template can be used to directly form a desired nanostructure.
Negative controls (template consisting of ultrapure water) were run for each gene.
Thus, a total of 37 reactions were studied per reference gene per tissue, including one negative control (template control without cDNA).
Rigorous assay characterization also required a standard control template.
The suspension was centrifuged and processed as described for DNA extraction for positive control template DNA.
PolyA+ submitted to reverse transcription without the actual RT enzyme were used as negative control template.
Control template strands contained unmodified dT at position X.
Positive control (template provided with kit) and negative control (heat inactivated samples) reactions were performed.
The clone named MF12 was used as the positive control template for PCR reactions.
Following brief centrifugation, 0.95 μL luciferase control template (Promega) was added to each tube.
Use of properly designed primers and control template DNAs can make PCR assays species specific.
