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By creating the single-stranded break, CtTopo I employ a mechanism called "controlled rotation" to relax supercoiled DNA, where one DNA strand rotates around the other [ 25].
A setup called Controlled Rotation of Liquids (CROL) has been developed and used to investigate rotational behavior of liquids interacting with solids under reduced gravity conditions.
DNA relaxation is supposed to proceed via a controlled rotation mechanism, in which the enzyme accompanies the end downstream of the cleavage site to rotate around the intact DNA strand [5].
The sample is suspended beneath an electronically controlled rotation stage in a reservoir of index matching fluid.
This equipment was designed to allow controlled rotation of the base plate within a range of ±15° via six links that were connected to the base plate area, which could move ±0.1 m in the vertical direction.
In our experimental setting, samples were positioned on a computer controlled rotation stage and scanned 180° around the vertical axis in rotation steps of 0.25 degrees at 40 kVp.
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At pre-defined events throughout the gait-cycle, the real-time system provided controlled rotations at constant speed of +/−5 degrees around the optimal trunk posture for each animal.
Curving her spine upward, she embarked on painstakingly controlled rotations; while her legs emerged from time to time, she transformed her body into an object both whimsical and sculptural.
We speculate that this was because while the pinch rotate mode is mainly used for Z axis rotation, it can also control rotation about the X and Y axes by simultaneously moving both fingers parallel to each other.
The TRANSP model found the new approach to be effective at controlling rotation.
Sauvage and collaborators in 1997 were able to control rotation in a [2]catenane through both electrochemical and photochemical means.
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