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This procedure reveals that the number of PS genes on the X chromosome is not significantly small (p = 0.25 when controlled for anatomical systems, and p = 0.32 when controlled for cell types).
On the other hand, we find a slightly significant deficit of PS genes on the Y chromosome (p = 0.015 when controlled for anatomical systems, and p = 0.05 when controlled for cell types).
Through the use of cytometer gating, we controlled for cell size and morphology (singlets vs budding cells).
To display the methylation data for both genes, we first obtained pyrosequencing data as to whether a particular CpG site was methylated (a threshold of 70% or higher methylation frequency was considered a methylated CpG site) in purified T cells that were controlled for cell number.
PC were controlled for cell type and concentration: some isolated mononuclear cells were observed A sample of 0.2 mm thick skin collected by means of a dermatome was placed in a transport tube containing 20 ml of DMEM, 10 U/ml of penicillin and 0.1 mg/ml of streptomycin sulfate.
While previous reports involving blood-based assessment of DNA methylation have controlled for cell mixture using complete-blood cell count (CBC) measurements [ 74, 75], such measurements are not capable of distinguishing between different lymphocyte subtypes and may be an oversimplification of the complexity and variability in circulating immune cells.
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Such interactions must be stringently controlled for cell-based tissue engineering and regenerative medicine therapies, and methods for studying and controlling cell cell interactions are being developed using both biomedical and engineering approaches.
Each firefly luciferase reading was normalised to its partner Renilla luciferase reading to control for cell number/viability and transfection efficiency.
To control for cell size variation between the mutant and wild-type cells, we quantified relative capsule size by determining the total cell diameter of cells with the surrounding capsule compared to the diameter of the cell itself [32].
We counterstained f-actin with Alexa-fluor 680-labeled phalloidin to control for cell numbers.
To control for cell synchronization, cells for the + N and –N conditions were harvested at the same time of day.
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