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On the following day (the first day of the experiment, Day 1), one group of grapevines was kept at 25/18°C day/night in the controlled environment room as the control.
Furthermore, host condition was additionally controlled as the colonies were kept in exactly the same conditions in the same controlled environment room.
Seed devitalisation was measured by germinating seeds in soil bins in a controlled environment room.
The laboratories at Rothera are very well equipped with, for example, analytical chemistry and microbiology labs, a controlled environment room, an aquarium, and a modern diving and boating facility.
Each individual was then returned to its container in the controlled environment room.
The plants were then moved to either a glasshouse (GH) or a controlled environment room (CER).
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Mice were housed in controlled environment rooms (72°F, ~40% humidity, 12 h/12 h in-phase light/dark cycles at 6 am/6 pm) in ventilated racks (Thoren; Hazelton, PA).
Plants were grown in controlled environment rooms (CERs) with an 8 hr/16 hr dark/light cycle, a constant 80% relative humidity and a light intensity of approximately 350 μmol m-2 s-1.
For analysis of flowering time and gene expression, plants were grown on soil in controlled environment rooms under LDs (16 h light 8 h dark) or SDs (8 h light 16 h dark).
Grapevine berries samples were taken from Vitis vinifera L. cv Cabernet Sauvignon rooted cuttings grown in controlled environment rooms with 16 h days and temperature regimes of either 30°C days and 25°C nights or 20°C days and 15°C nights.
Responses to vernalization from 126 Arabidopsis accessions were obtained from published data [ 22] and analyzed by subtracting days to flowering with vernalization from days to flowering without vernalization (plants grown in controlled environment rooms with 16 hour light).
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