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It is maintained on a controlled copy basis, with manual holders receiving copies of new or revised pages as they are issued and notices to remove deleted pages.
Retroviral (both γ-retroviral and lentiviral) vectors are attractive for gene therapy of genetic diseases because of efficient and stable transgene insertion with a controlled copy number11 However, in both mice and men, leukemias have been reported in which insertional activation of a cellular proto-oncogene by integrated retroviral vectors represented the initiating event (Figure 1).
When using low multiplicities of infection, retroviral transduction results in the delivery of a controlled copy number of the sequence encoding the effector molecule.
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They could only do it by giving it to the most prestigious distributors, and allowing the distributors to sell carefully protected and controlled copies of the food to people who could afford it.
We describe here construction details for MW005 and demonstrate its use in supporting controlled copy-number induction of oriV-equipped fosmid and BAC genomic clones.
Controlled copy-number regulation requires the vector be equipped with the oriV replication origin and be propagated in a suitable E. coli host that carries an inducible copy of the trfA gene encoding the trans-acting replication protein TrfA.
On the other hand, the discrepancies observed between the two elements may be accounted for by the different factors that control copy numbers in each of these elements.
However, thus far a way of controlling copy number and integration sites has not been available, leading to variable transgene expression caused by position effects.
Freelancers, on the other hand, are unhappy with what they regard as the erosion of their right to control copying, which they see, for example, in the legally sanctioned practice of posting "snippets" on sites like Amazon, iTunes, and Google Books.
Conversely, a strain overexpressing TPO1 (oex TPO1), created by genomic integration of a second, GPD1 promoter controlled TPO1 copy, arrested for a longer period (Fig 1A).
There was a weird dream about an out-of-control copy machine.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com