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Microbeads coated with either human E-cadherin or with anti-HLA antibody (as a cell binding negative control) were attached to A431 cells expressing PAPC, FLRT3 or both, and cells were washed, fixed and subject to double immunofluorescence staining: red fluorescence for E-cadherin cytoplasmic domain (not present in the ligand) and green fluorescence for either PAPC or FLRT3 (Figure 4A).
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To avoid potential positional effects, experimental and control masses were attached in alternating order along the pipe.
For deployment in the field, 6 experimental and 6 control egg masses were attached to a 2-m piece of PVC pipe with holes drilled at 10-cm intervals.
For the pull down assay, GST as a negative control and GST-hsc70 were attached to glutathione columns.
On the control scaffold, a little scattered ECs were attached to the microporous face at day 7.
To control for disclosure risks, geographical data were attached to the postal codes using a third party (the Health Informatics Centre, Dundee) and the postal codes were removed from the final research dataset.
Temperature data loggers were attached to blank controlled intrauterine drug-release devices (CIDR; contained no active compound) that recorded vaginal temperatures every 5 min for the experiment; vaginal temperatures were then averaged for every hour before data analysis.
Disconnect the cables from the remote control taking notes as to how they were attached.
That is, multiple channel absolute/relative velocity feedback controllers, their hybrid combination and an existing passive control system to which the former control systems are attached in order to improve overall control efficiency.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com