Similar(60)
Cell Viability (CV) in the IS control well was set as 100%%.
Control well was treated with equivalent volume of celecoxib-free media.
Checkshot from the control well was used to associate travel time (a measure of velocity) with each of a number of depths in the wells.
The control well was untreated.
For each subject cytokine concentrations in the unstimulated control well were subtracted from TB specific responses, expressed as pg/ml.
The result was interpreted as indeterminate if the number of spots in the positive control well was less than 20 and if the antigen specific wells were negative.
One control well was added only DMEM/F12 SFM.
A control well was inoculated with sterile BHI medium.
The absorbance (au) from the water control well was subtracted from all 95 substrate-containing wells.
A control well was also set up with solvent only, that is, DMSO as negative control.
The adjusted OD of each control well was then assigned a value of 100% growth.
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