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As a polyester positive control was included in every prepared plate, the sample size for polyester was n = 9.
A non-inoculated control was included.
For each PCR set, a negative control was included.
Each assay was carried out in triplicates and a non template negative control was included.
In order to control nuclease contamination, negative control was included at every reaction.
A no template control was included.
An ethanol solvent control was included in the assay.
A non-template control was included for all PCR reactions.
A non-template control was included for each gene.
A negative control was included in all co-transfections.
A no antibody control was included as well.
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