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This airstream was composed of a carrier stream (1.75 l min-1) and an odor stream (0.25 l min-1) that was directed from a mineral oil control vial to an odor vial by a trigger-controlled solenoid valve.
The matured nauplii were applied to each of all experimental vials and control vial.
The 0 min PVS2 control vial was transferred directly into water bath for 2 min for thawing without sucrose solution.
A positive result was defined by ≥50% cell lysis with no evidence of cytotoxicity in the relevant antitoxin control vial.
When the GI in the drug vial decreased in relation to the GI of the control vial, the organism was considered "susceptible".
Conversely, when the GI of the drug vial increased in relation to the GI of the control vial, the organism was considered "resistant".
Similar(49)
In the control vials same volumes of DMSO (as in the sample vials) were taken.
Each experiment included control vials that lacked redox mediators, as well as blank vials that contained media and inoculum but lacked switchgrass or redox mediators.
Control vials were inoculated with 70 µl sterile PBS/5% sucrose.
For the 4 day experiments (Figures 1, 2), flies were initially placed in control vials, and then at 21 hours the flies were transferred to control vials or vials adjusted to the indicated H2O2 or TBHP concentrations.
For DAM experiments lasting 5 weeks (Figure 4), vials containing Drosophila media were adjusted to 64 µg/ml doxycycline (DOX) plus 64 µg/ml ampicillin, while control vials were adjusted 64 µg/ml ampicillin alone.
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