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Albumin secretion was analysed in parallel as a control viability and tissue specific secretory parameter.
Whilst BSA showed a low inhibition of cell viability of BV-2 cells overall, with about 70% compared to the control (viability taken as 1), the viability did decrease with increasing concentrations of mBSA (0 15 μmol/L) (Fig. S6B).
These include 30 genes that control viability in the fruit fly.
Compared to the medium control, viability was reduced by 47.86% (n = 4, P = 0.0286).
The viability of treated cells were calculated similarly from the absorbance values and expressed as percentage of control viability.
Thus, these findings illustrate the distinct modes by which aak-2 functions to control viability during L1 and dauer diapauses.
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Using MTT assay, HMGB protected against hypoxia-induced cell death vs controls (viability 75% vs 61%, P < 0.05).
An siRNA to luciferase was used as a negative control (100% viability) and a no cell control was used a 0% viability control.
After the treatment of samples and positive control, cell viability was detected and a cell growth curve was graphed.
As an additional control, the viability of cells seeded on membranes, however kept in submerged conditions, were similar to the incubator controls of cells lifted to the ALI. Figure 5 Mainstream CS exposure of cells.
1% Triton X-100 was added to untreated cells as a negative control for viability.
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