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The control vector was a replication-defective lentivirus encoding GFP (Lenti-GFP).
These values were then normalized against the mean value of the control (control vector).
The following plasmids were injected: control vector, RHEB-WT, RHEBp.P37L and RHEBp.S68P.
The latter one is considered as a control vector.
HBV-infected HK-2 cells were simultaneously transfected with Lsd1 shRNA and full-length Tlr4 or control vector.
A similar non-endogenous control vector, pFGC-egfp2, was constructed using the primers eGFP-F/eGFP-R (Table 1 29.
The control vector is an EGFP expression transcript in the pReceiver-Lv156 lentiviral plasmid system (Cat# Ex-EGFP-Lv156).
(d,e) Percentage of donor derived LSK HSCs transduced with shRNAs against Nap1l3 or a control vector.
Overexpression of the RHEB mutant proteins caused an increase in soma size, confirming that these mutations do not cause a loss of function (Fig. 3a, b, one-way ANOVA, p < 0.0001, F 3,260) = 50.35; control vector vs. RHEB-WT: p < 0.0001; control vector vs. RHEBp.P37L: p < 0.0001; control vector vs. RHEBp.S68P: p < 0.0001 by Tukey's multiple comparisons test).
(a) qPCR analysis of Nap1l3 mRNA levels (normalised to Hprt) of sorted LSK HSCs transduced with an shRNA against Nap1l3 (Nap1l3 shRNA), or a control vector (SC shRNA).
SCOP overexpression levels were ~2 fold higher in uninjured DIV10 neurons transduced with a SCOP overexpressing vector vs. uninjured neurons transduced with a control vector.
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