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Control tubes without tested samples were essayed simultaneously.
The control tubes were prepared and incubated under the similar conditions.
Both inoculated and control tubes were incubated for 24 h at 30 °C.
Simultaneously, control tubes without nanoparticles but same amount of inoculums and without inoculums and nanoparticles were also run.
The absorbance of both the test and control tubes was measured at 530 nm against the blank (without tannic acid).
Control tubes were incubated with same concentration of heat denatured enzyme along with substrate and methanolic rhodanine, blank was made without enzyme.
Control tubes lacking either AxV-FITC or PI, or both, were included to complete the controls.
Subsequently, twenty µl of chloroform was added to the control tubes to lyse the bacteria.
Negative control tubes were stimulated with PBS in place of Salmonella.
Extraction negative control tubes were always processed in parallel with tubes containing samples.
Background responses detected in negative control tubes were subtracted from those detected in stimulated samples for every specific functional combination.
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