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The negative control tube did not contain any inoculum, and positive control tube was free of antibacterial agent.
A no-peptide (control) tube was also included in each run.
The control tube contained sucralose but was totally covered with aluminum foil to prevent light exposure.
Thus, a single positive control tube does not determine if all PCR reactions worked properly.
Blue and redhistograms represent the indicated first and second bacteria mixture counts, respectively, in the control tube.
All PCR reactions were run in triplicate in the presence of a blank control tube.
A control tube with sterile water (Sigma) in place of template was always included.
A control tube without mitomycin C was added to evaluate the presence of 'non-drug-induced' bacteriophages.
The enzymatic activity was defined as units per milliliter: 200× [(OD420 of the culture - OD420 in the control tube)/minutes of incubation] × dilution factor.
Hence, the final quantification value for each condition indicates the relative change of gene expression in the problem tube with respect to the control tube, for each sample.
Amplification of P. falciparum DNA yielded sigmoid shaped amplification curve while the control tube (no DNA) had no measurable fluorescence indicated by a flat line in the plot.
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