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After 1567 he became control tester of coins at Kutná Hora, near Prague.
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In the gaming room, where executives and quality-control testers gather for daily bouts, it is a different story.
The assembly line is mostly automated, with machines placing and soldering components onto circuit boards before feeding the boards to human quality-control testers.
In short, the cDNAs from driver (control) and tester (As-treated) samples were digested with endonuclease RsaI and the tester cDNAs were ligated to two different adapters at either end of the cDNAs separately [ 52].
Subtractive hybridization was performed using five-times more driver (control) than tester (infested) cDNA.
The PCR not only exponentially amplifies the control and tester DNA but also separates them from each other.
The adapter-linked control and tester DNA are reassociated through mixing at a ratio of 1 1, denaturing and annealing.
Briefly, the control and tester cDNA's are digested with Taq I restriction enzyme, then ligated with the CT and TT adapters, respectively.
The DNA reassociation results in the quadratic magnification of expression ratios for the up- and down-regulated genes in control and tester samples.
With indirect labeling, aminoallyl-dUTP is incorporated into control and tester DNA with PCR of 3 cycles after the reassociated DNA templates are amplified.
For example, the 3DNA fluorescent dendrimer probes (Genisphere, Montvale, NJ) can be attached on the CT and TT primers which then are used to amplify the control and tester DNA's.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com