Exact(42)
To this end, the paper first describes generic vertical and horizontal management control templates: archetypes of management control.
RNA ligation reactions were used as control templates to indicate the absence of DNA contamination.
Noteably, MRE11 was detected in the DSwt, but not in any of the control templates lacking telomere repeats.
Furthermore, IHNV, VHSV, IPNV, KHV, NDV, AIV (H5, H7, H9), CO cell, EPC cell, FHM cell, eggs of sturgeon and salmon, tissues (muscle, pancreas, gill, spleen, fin, brain, kidney) of ornamental carps, common carps, goldfishes, and grass goldfish were used as control templates to verify the specificity of the method.
Both reference and test Scorecard RNA contained one set of calibration control templates serially diluted cYIR01-cYIR100), and one set of ratio control templates (rYIR1-rYIR8).
Gene-specific plasmids served as control templates with specific oligonucleotide primer pairs.
Similar(18)
Thus, a total of 37 reactions were studied per reference gene per tissue, including one negative control (template control without cDNA).
Rigorous assay characterization also required a standard control template.
The suspension was centrifuged and processed as described for DNA extraction for positive control template DNA.
PolyA+ submitted to reverse transcription without the actual RT enzyme were used as negative control template.
Control template strands contained unmodified dT at position X.
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