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To distinguish autofluorescent cells from cells expressing low levels of individual surface markers, we established upper thresholds for autofluorescence by staining samples with fluorescence-minus-one (FMO) control stain sets [15].
Fluorescence minus one (FMO) and isotype control stain sets were used to assess detection thresholds.
The Multi-Cytokeratin (AE1/AE3) was used as a control stain for the identification of tumor cells.
A control stain was performed without primary antibodies in order to confirm that there was no occurrence of non-specific binding.
Caveolin-1 staining of peripheral endothelial cells and nephric fat within the sections was monitored and served as positive internal control stain for immunoreactivity.
Test larvae were produced by mating males from RNAi line with females of the GAL4 strain to drive RNAi, while control samples were obtained by crossing males of the control stain to females of the GAL4 strain.
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(D) IPPE control stained with anti-TSPO.
Gating for all sorts was defined by isotype control staining.
Negative control staining was performed by omitting the primary antibodies.
Figure 8 Immunohistochemistry. (A) IPPE control stained with anti-CD68.
Open area represents staining with JL2 and shaded area represents isotype control staining.
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