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Negative control slides were stained with non-immune rabbit IgG and secondary antibodies as above (data not shown).
Control slides were treated with non-immune rabbit serum and FITC-conjugated goat anti-rabbit IgG.
Negative control slides were only incubated with the secondary and tertiary antibodies.
For control slides primary antibodies were omitted.
Positive and negative control slides were included in each batch.
Negative control slides were performed with the primary antibody omitted.
For negative control slides, primary antibody step was omitted and only antibody diluent applied instead.
Negative control slides received identical preparations for immunostaining, except that primary antibodies were omitted.
Negative control slides were incubated with the same labeling solution without terminal transferase.
Control slides were incubated with mouse IgG2a (Sigma), the isotype of all primary antibodies.
Control slides were incubated with a nonimmune serum instead of primary antibody.
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