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While transcription is a major control point of gene expression, a gene's transcript can also be subject to regulation at the levels of RNA processing, transport, localization, translation, and degradation.
DOI: http://dx.doi.org/10.7554/eLife.08504.002 Transcription initiation is a major control point of gene expression.
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Expression quantitative trait loci (eQTLs) represent genetic control points of gene expression, and can be categorized as cis- and trans-acting, reflecting local and distant regulation of gene expression respectively.
The use of linkage analysis in combination with genome-wide expression profiling by microarray, also known as 'genetical genomics' [1], enables the genetic control points of gene expression to be mapped to the genome.
While this is a simplified model, it is a useful framework for establishing the control points of basic gene expression.
Genetic intervention in jatropha requires understanding of the biosynthetic pathways, metabolic flux control points, cloning of the genes that code for the enzymes and proteins involved in the metabolic pathways and development of molecular markers.
Considering the significance of bradyzoite formation for the pathogenesis of T. gondii, it is very likely that the control of bradyzoite differentiation is a multigenic phenomenon with more than one control point, and therefore disruption of a single gene is unable to completely abolish this process.
For the second gene Ascl3, Figure 1b, ESS++ also finds a single cis-acting genetic control point (SNP J697407) for the variation of the gene expression in all seven tissues, highlighting the fact that the second trans-acting locus found in Petretto et al. (2010) was specific for the four tissues considered (adrenal gland, fat, heart, kidney).
Pre-mRNA processing is an essential control-point in the gene expression pathway of eukaryotic organisms.
The median value of all control plant data points of a gene was used to represent the gene expression level.
Expression of genes encoding ACC synthase, a key control point for ethylene and ripening in tomato, was also highly variable.
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