Sentence examples for control over contamination from inspiring English sources
A limitation of the study was the lack of control over contamination by other QI efforts.
PCR was performed by adding 1 U of heat-labile Uracil-DNA Glycosylase (USB) and an initial incubation step of 15 min at 37°C to control for carry-over contamination.
Surgery – often referred to as surgical source control – is essential and generally considered to be a decisive factor for the outcome in patients with IAI.[ 2– 4] Source control is defined as the elimination of the source of infection and the control over ongoing contamination.
In this study dTTP was replaced with dUTP in all protocols in order to identify a sensitive PCR test that also includes a control measure against carry-over contamination.
Molecular-grade water was used instead of blood as an extraction control for checking for carry-over contamination in every run of DNA extraction and PCR amplification.
Controls without DNA template were included in all PCR runs to exclude any cross-over contamination.
One positive (DNA of Aporrectodea spp., Lumbricidae) and one negative control (PCR-grade water) were included within each PCR run to check for amplification success and DNA carry-over contamination.
This prevents carry-over contamination.
Yet, the few examples of horizontal spread in the laboratory environment confirm the existence of strategies to overcome human-imposed control over homogeneity, multiplication and cross-contamination.
Stringent anti-contamination precautions to control PCR contamination were undertaken in the laboratory environment.
