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As a negative control, omission of primary antibodies was used.
All the RT-PCRs included a negative control (omission of template) and a positive control (Synechocystis gDNA).
Positive control (section from a pancreatic cancer) and negative control (omission of primary antibody) were included in each run.
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There are no air bags, antilock brakes or stability control — omissions permitted for low-volume manufacturers.
A simultaneous examination of negative controls (omission or blocking of primary antibody with antigenic peptide confirmed the absence of nonspecific immunofluorescent staining, cross-immunostaining, or fluorescence bleed-through.
In every study the analysis of negative controls (omission of primary antibody) was simultaneously performed in order to exclude the presence of non-specific immunofluorescent staining, cross-immunostaining, or fluorescence bleed-through.
The analysis of negative controls (omission of primary antibody) was simultaneously performed in order to exclude the presence of non-specific immunofluorescent staining, cross-immunostaining, or fluorescence bleed-through.
Known positive and negative controls (omission of the primary antibody) were used in every run.
For antibody controls, omission of the primary antibody and anti-isotype antibodies were also employed.
Negative controls (omission of the primary antibody) were included in each run.
These bands were not present in the negative controls (omission of primary antibody).
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