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Tight control of transgene expression in a cell line stably expressing CymR is evident by examining GFP expression in 293-CMV5-CuO-GFP 293-CMV5-CuO-GFP 293-CMV5-CuO-GFP 293-CMV5-CuO-GFP
The control of transgene expression is vital both for the elucidation of gene function and for the engineering of transgenic crops.
Precise control of transgene expression is pivotal to the engineering of plants with increased disease resistance.
However, accurate spatiotemporal control of transgene expression is still difficult to achieve.
The precise control of transgene expression is essential for biopharmaceutical manufacturing, gene therapy and tissue engineering.
We used QF, QS and quinic acid to achieve temporal and spatial control of transgene expression in various tissues in C. elegans.
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These results suggest that the vector dose can be used to control the level of transgene expression.
For example, a number of housekeeping genes should be satisfactory controls for comparisons of transgene expression level in the same tissue type from different transgenic lines grown under identical conditions.
In addition to temporal control, the Gene-Switch system allows for control of the magnitude of transgene expression.
Their flexible design facilitates the accommodation of sophisticated elements of control for the precise tuning of transgene expression.
The main advantage is that they can provide control over the timing and location of transgene expression.
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